Tiffiney R. Hartman, Daniel Zinshteyn, Heather K. Schofield, Emmanuelle Nicolas, Ami Okada, Alana M. O'Reilly (2011) CIL:13748, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell. CIL. Dataset
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In the absence of boi (boi[e] mutant), Hedgehog (Hh, red) is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13744, 13745). Expression of Boi in apical cells relocalizes Hh to apical cells (CIL#13746). Apical cell expression of Ihog, which also binds Hh directly, rescues Hh localization in the boi[e] mutant (this image; boi[e]; ihog[EP]/+; babGal4/+). Germ cells are identified with anti-Vasa (blue). Image is Fig 3E in J Cell Biol. 2010. 191: 943-952. Images in Fig 3 include CIL# 13744, 13745, 13746, 13747, 13748.
在boi(boi[e]突变体)缺失的情况下,Hedgehog(Hh,红色)从顶细胞重新分布至局部毛囊干细胞微环境(CIL# 13744,13745)的细胞外空间。顶细胞中Boi的表达使Hh重新定位至顶细胞(CIL#13746)。Ihog(也直接结合Hh)在顶细胞的表达通过挽救boi[e]突变体中的Hh定位而发挥作用(本图;boi[e];ihog[EP]/+;babGal4/+)。通过抗Vasa抗体(蓝色)识别生殖细胞。图像为J Cell Biol. 2010年191期第943-952页中的图3E。图3中的图像包括CIL# 13744,13745,13746,13747,13748。
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