Identification of new RhoGDI2 interactants by co-IP and MS

A co-precipitation experimental model was developed to identify proteins potentially physically associated with RhoGDI2. Cells expressing RhoGDI2 tagged with FLAG® M2 were generated. This tag was placed at either the C- or N-terminus to reduce the probability of missing potential partners specific to the N- or C-terminal end domains of RhoGDI2. Tagged RhoGDI2 was immuno-precipitated with anti-FLAG® M2 antibody and co-precipitated proteins were identified by Mass Spectrometry.