RNA-seq of 2D-culture balding and non-balding dermal papilla cells

Androgenetic alopecia (AGA, male patterned baldness) is a prevalent hair loss condition in males that develops due to the influence of androgens and genetic predisposition. With the aim of elucidating genes involved in AGA pathogenesis, we cultured immortalised balding human dermal papilla cells (BAB) and immortalised non-balding human dermal papilla cells (BAN) in 2-dimensional culture format, and compared gene expression using RNA-seq between BAB and BAN cells. Examination of transcriptome difference between immortalised balding human dermal papilla cells (BAB) and immortalised non-balding human dermal papilla cells (BAN) under 10nM DHT treatment, technical triplicate.