Metagenomic shotgun sequencing of healthy stool bank donors and multiple recurrent Clostridioides infected recipients from the Netherlands Donor Feces Bank
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Fecal shotgun metagenomes of healthy donors from the Netherlands Donor Feces bank and of multiple recurrent Clostridioides infected patients treated by fecal microbiota transplantation. Note that for privacy reasons, human-derived reads have been removed from these files by mapping raw reads to the human reference genome (GRCh38.p7 (https://www.ncbi.nlm.nih.gov/assembly/GCF_000001405.33/)) using bowtie2 (version 2.4.2) with the '--very-sensitive-local' parameter. Non-mapping reads are selected with samtools (version 1.11) using the parameters ' -f 1 -f 4 -f 8'. The resulting paired-end raw read files are deposited here. This publication first described the dataset: https://doi.org/10.1053/j.gastro.2021.06.009 The human read removal workflow is based on: https://git.lumc.nl/snooij/metagenomics-preprocessing



