Laser illumination of live embryos expressing CactDN-psd induces transient cyclical nuclear accumulation of Dorsal-GFP (low mag).

The movie shows an embryo expressing Dorsal-GFP together with CactDN-psd from nc12 up to nc14. In brief, embryos were imaged under two conditions: (i) first, imaging was initiated at nc12 using the low power 488nm laser; (ii) after mitotic division (nc13), high power 488nm laser light was applied for a period of 20min. Importantly, high power blue light (488nm) illumination initiated degron-mediated loss of CactDN-psd activity during nc13, resulting in transient Dorsal-GFP nuclear localization just before the onset of nuclear mitosis. The movie is a compilation of these 2 imaging sessions taken over time. Snapshots from the movie are shown in Fig 10A–10A“‘.
(ZIP)