Diet-specific circadian transcriptome of Canton-S and Tim01 females.

This study aims to elucidate nutrient dependent changes to the circadian transcriptome of whole flies. In particular, we aim to identify how dietary restriction influence circadian transcriptional output. Circadian analyses were performed to invesitigate diet-dependent changes in the number of circadian transcripts as well as their phases and circadian amplitude. We performed a time-resolved analysis of transcript level expression from whole-fly lysates of adult Canton-S and Tim01 mutant flies reared on nutrient rich (ad libitum, 5% yeast extract) or dietary restriction (0.5% yeast extract) diets. Microarrays were performed every four hours over the course of a day (ZT 0, 4, 8, 12, 16, 20). Prior to collection, flies were reared on AL or DR diets for seven days in a 12:12h light-dark cycle at 25°C.