Detoxification of aflatoxin B1 by essential oils of medicinal herbs
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The essential oils (EO) extracted from the medicinal plants viz., Heliotropium bacciferum, Ocimum dhofarense and Zataria multiflora were diluted with methanol (1:10, v/v) and 250 μl of EO was mixed with AFB1 (50 μg/l) in a microcentrifuge. Following a 24-h incubation at 25°C, AFB1 in the mixture was extracted with 250 μl of chloroform. The chloroform fraction was evaporated at 60°C using a water bath and the residue was quantified by enzyme-linked immunosorbent assay using a commercial kit (RIDASCREEN Aflatoxin B1; R-Biopharm AG, Darmstadt, Germany).
创建时间：
2021-10-04
