ChIP-seq of histone H3
收藏NIAID Data Ecosystem2026-03-10 收录
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资源简介：
After fixation with 1% of formaldehyde and quenching with 125 mM glycine, cells were disrupted by shaking with glass beads (11079-105, BioSpec) in a Multi-Beads Shocker (MB400U, Yasui Kikai, Japan). A Bioruptor (UCD-250, Cosmo-bio, Japan) was used for DNA shearing. For immunoprecipitation, antibody against histone H3 (abcam, ab1791) was used. QIAquick PCR Purification Kit (28106, QIAGEN) was applied to purify immunoprecipitated DNA. Sequencing was done at BGI (http://www.genomics.cn/en/).Accession of input DNA is DRP000890.
创建时间：
2017-09-17
