ChIP-seq of H3K9me2 levels in wild type and deletions of tor1 or gad8 in Schizosaccharomyces pombe

We report that loss of TOR complex TORC2 and Gad8 (AKT in human cells) results in loss of gene silencing at the subtelomeric regions. We demonstrate that TORC2-Gad8 is required for assembly of specific heterochromatic regions that lie between the chromosome arm euchromatin and telomere-proximal heterochromatin. At these subtelomeric regions, loss of TORC2-Gad8 results in loss of the heterochromatic histone 3 lysine 9 di-methylation (H3K9me2).