Tissue damage score following recovery of FLBZ-exposed B. malayi females from naïve jirds.

A, B. Worms incubated for 24 hr prior to transplantation and recovered from jirds for 5 days, 4 weeks or 8 weeks after. Data is a combination of two experiments. In experiment one worms transplanted into each of three jirds for all treatment groups were maintained for 5 days or 4 weeks. Worms transplanted into each of three jirds per treatment group in experiment two were maintained for 4 weeks or 8 weeks. The 4 week data were combined for presentation purposes. A minimum of 25 worms were assessed histologically C, D. Worms incubated for 6, 12, or 24 hr prior to recovery at 8 weeks. Worms were transplanted into each of three jirds per treatment group, following the appropriate duration of in vitro exposure to FLBZ. The number of worms assessed histologically ranged from 11 to 39. Damage scored as minor (1), moderate (2), severe (3), or no damage (0) by two methods: A, C. Individual section scoring method; B, D. Classical histopathological survey method.