Single-cell RNA sequencing of human kidney

A
comprehensive cellular anatomy of normal human kidney is crucial to address the
cellular origins of renal disease and renal cancer. Some kidney diseases may be
cell type-specific, especially renal tubular cells. To investigate the
classification and transcriptomic information of human kidney, we performed a
method to obtain single-cell suspension of kidney rapidly, and conducted
single-cell RNA sequencing (scRNA-seq). We present scRNA-seq data of
23,366 high quality cells from human kidneys of 3 donors. In this dataset, we
show 10 clusters of normal human renal cells. Due to the high quality of single
cell transcriptomic information, proximal tubule (PT)
cells were classified into 3 subtypes and collecting ducts cells into 2
subtypes. Collectively, our data will provide a reliable reference for the
studies of renal cell biology and kidney diseases.