Active site of cyanobacterial RNA polymerase compensates for the absence of proofreading factors [Ecoli]

We found that cyanobacterial RNA polymerase possesses very efficient intrinsic proofreading ability. This ability allows model species of cyanobacteria, Synechocystis sp PCC 6803 to keep in vivo level of transcriptional mistakes close to that of E.coli. Overall design: Sequencing of total RNA prepared from exponentially grown cells