The binding of C1q to THP-1-derived macrophages was inhibited by r<i>T</i>s-Pmy.

(A) After fixing with 4% paraformaldehyde and blocking with goat serum, M2 type macrophage cells were incubated with C1q, C1q plus rTs-Pmy or PBS alone for 1 h at 37°C, and then detected with an anti-C1q mAb or anti-Ts-Pmy mAb 9G3. Dylight 488 was used as a secondary antibody (green). The nuclei were stained with DAPI (blue). The imagine magnitude is 400 X with one cell 1000 X at the bottom right corner. (B) The fluorescence intensity was measured with high content analysis. The results shown are means ± SD and are representative of three independent experiments. ***p<0.001.