HIV-1 NL4-3 Gag mutants selected for testing of predicted energy costs (E) by an in vitro HIV-1 replication capacity assay.

aEnergy cost predicted by original Ising model [11] taking into account differences in NL4-3 and the multiple sequence alignment used in model generation. E is 2.98 for wild-type NL4-3 p24 and 3.43 for wild-type NL4-3 p17.bMutation pairs within an immunologically vulnerable group of co-evolving residues, termed sector 3, that we previously identified qualitatively [12].cE>90 or E = ∞ were considered high E values and E