Expression data from Triticum aestivum near isogenic lines (TugelaDn0, RWA susceptible; TugelaDn1, RWA resistant; TugelaDn2, RWA resistant; TugelaDn5, RWA resistant) after infestation by Diuraphis noxia biotype SA1

The wheat Nils expression profiles differ because of their response to aphid feeding (i.e., antibiosis - Dn1; tolerance - Dn2; antixenosis and antibiosis - Dn5) We used GeneChip Wheat Genome Array (Affymetrix, USA) analysis to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. Plants of each cultivar were infested with 10 aphids and incubated for 48 h. All leaves except the first leaf were harvested into liquid N2 and stored at 280°C prior to RNA isolation. Data analysis was conducted using CranBioconductor and using a linear mixed statistical model (Botha et al. 2014, Biology Open (2014) 3, 1116–1126 doi:10.1242/bio.201410280).