Single Cell Smart-Seq 3 RNA-Seq and Bulk Exome Seq from Breast Cancer Patients

<b>Data Set Description</b><b><br></b>Single cell RNA sequencing (Samrt-Seq3) and Whole exome sequencing from multiple regions of individual tumors from Breast Cancer patients and also single cell RNA seq for two ovarian cancer cell lines.<br>The dataset contains raw sequencing data for various high-throughput molecular tests performed on two sample types: tumor samples from two breast cancer patients and cell lines derived from High-grade serous carcinoma Patients. <br><br>The breast cancer data comes from two patients: patient 1 (BCSA1) has two tumor regions A-B and patient 2 (BCSA2) has five regions(A-E). For a normal sample and each region from each patient Whole Exome Sequencing was performed using Twist Biosciences Human Exome Kit by the SNP&amp;SEQ Technology platform, SciLifeLab, National Genomics Infrastructure Uppsala, Sweden. Also for each patient, EPCAM+ CD45- sorted cells from all the regions where sorted to a 384 well plate, and Smart-Seq3 libraries were prepared at Karolinska Institutet and sequenced at National Genomics Infrastructure Uppsala, Sweden.<br>The HGSOC cell-line data comes from OV2295R2 and TOV2295R cell lines described in Laks et al <br>Cell 2019 Nov 14; 179(5): 1207–1221.e22 doi: 10.1016/j.cell.2019.10.026 . The cell line Smart-Seq3 libraries were prepared from two 384 well plates at Karolinska Institutet and <br>sequenced at National Genomics Infrastructure Uppsala, Sweden.<br><b>Terms for access</b>This dataset is to be used for research on intratumor heterogeneity and subclonal evolution of tumors. To apply for conditional access to the dataset in this publication, please contact [e-mail].<br><br><br>