Indel profiling of primary hepatocytes by deep sequencing. Indel profiling of primary hepatocytes by deep sequencing

CRISPR/Cas9 has proven to be an efficient tool for gene editing both in vitro and in vivo. The aim of this study is to evaluate editing efficiency of various genes targted in Rosa26-Cas9 knock-in mice. Overall design: Efficiency of in vivo genome editing was assessed using for hepatocytes isolated from the livers of Cas9-EGFP mice to which adeno-assocaited viruses (AAVs) packaged with sgRNAs targeting various genes were injected. Genemic regions containing each cut site were amplified by PCR, and libraries were prepared for deep sequencing.