Nutritional requirements of <i>T</i>. <i>gondii</i> predicted <i>in silico</i>.

Required precursors: 1—phenylalanine or phenylpyruvate; 2—folic acid or its derivatives; 3—l-2-aminoadipate 6-semialdehyde; 4–3-methyl-2-oxobutanoic acid; 5–4-methyl-2-oxopentanoate; 6 –(s)-3-methyl-2-oxopentanoic acid; 7—l-homocysteine; 8—phenylpyruvate; 9—a source of purine moiety: hypoxanthine, adenine, adenosine or similar; 10—pantothenate or valine+β-alanine; 11—riboflavin; 12—nicotinic acid or its derivatives; 13—thiamine; 14–4-hydroxybenzoate; 15—an undefined”sulfur donor” molecule; 16—lipoic acid; 17—choline; 18—myo-inositol. Nutritional requirements of T. gondii predicted in silico.