Diffusion coefficients obtained by FCS measurements (Figure 2) and geometrical properties of MalE2 molecule (see Materials and Methods section for more details).
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(1)Errors on FCS measurements.(2)Molecular radius (RSph calculated from the D1 diffusion coefficient using equation 3).(3)Prolate ellipsoid semi-axes (a, b) and corresponding diffusion coefficient (DE) calculated using Equation 4, assuming the same spherical volume obtained at pH 7.0 (i.e. ≈ 1·105 Å3).
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2015-12-02
