Identification of the cell subset generating crescents within human parietal epithelial cells (PECs)

We generated a single-cell RNA sequencing (scRNAseq) dataset of primary cultures of human glomerular parietal epithelial cells. Comparing this dataset with an already available dataset obtained by scRNAseq of human renal progenitor cells (GSE137620), we identified an immature PEC subset characterized by the expression of stem cell markers and stratifin (SFN). Moreover, we analyzed the effect of treatment with the HDAC inhibitor panobinostat on PEC transcriptome demonstrating the upregulation of podocyte-specific markers in different PEC subsets including the stratifin-expressing subset. Identify and characterize an immature PEC subset and its response to panobinostat treatment