Genetic analysis of SnRK1β3 subunit of peach and the functional identification of overexpression transformed tomato

The sucrose non-fermentation-related kinase 1 (SnRK1) protein complex in plants plays an important role in energy metabolism, anabolism, growth, and stress resistance. This protein complex typically consists of an α subunit, three β subunits, one or two βγ subunits, and a γ subunit. Studies on plant SnRK1 have primarily focused on the functional α subunit, with the β regulatory subunit remaining relatively unexplored. The present study aimed to elucidate the evolutionary relationship, structural prediction, and interaction with the core α subunit of peach SnRK1β3 (PpSnRK1) subunit. In addition, we produced transgenic tomato plants overexpressing PpSnRK1 (OEPpSnRK1). We mainly tested the growth index and drought resistance of transgenic tomato plants.The results showed that PpSnRK1 has a 354 bp encoded protein sequence (cds), which is mainly located in the nucleus and cytoplasm. Phylogenetic tree analysis showed that PpSnRK1β3 has similar domains to other woody plants. Transcriptome anal..., Phylogenetic tree analysis
 
Protein sequences of SnRK1β3 were gathered from the NCBI database for 14 plant species ([https://www.ncbi.nlm.nih.gov/](https://www.ncbi.nlm.nih.gov/)), which included *Arabidopsis thaliana*,* Populus trichocarpa*,* Prunus persica*,* Vitis vinifera*,* Malus domestica*,* Prunus avium*,* Ananas comosus*,* Nicotiana attenuata*,* Carya illinoinensis*,* Durio zibethinus*,* Mangifera indica*,* Pyrus x bretschneideri*,* Triticum aestivum*, and *Gossypium hirsutum*. These sequences were analyzed using MEGA7 software to construct an evolutionary tree using the neighbor-joining method with 1000 bootstrap copies [1]. Structural domains were analyzed using Pfam ([http://pfam-legacy.xfam.org/](http://pfam-legacy.xfam.org/)) [2]. Protein sequence alignment was performed using DNAMAN software [3].
 
Yeast two-hybrid (Y2H) assay
 
The PpSnRK1α cds was cloned into the PGBKT7 vector, and the cds of PpSnRK1β3 was cloned into the PGADT7 vector (Primers: Table S2). These two vec..., , # Genetic analysis of SnRK13 subunit of peach and the functional identification of overexpression transformed tomato

[https://doi.org/10.5061/dryad.6djh9w19t](https://doi.org/10.5061/dryad.6djh9w19t)

## Description of the data and file structure

The files uploaded for this assay include sequencing files (*b3-bifc.ab1*, *a-bifc-2.ab1*, *a-nluc-1.ab1*, *b3-cluc.ab1*, *a-nluc-2.ab1*, *a-bifc-1.ab1*, *a-BD-1.ab1*, *a-BD-2.ab1*, *b3-AD.ab1*,*b3-GFP.ab1*), sequence files (*CDS-α-DNA.fa*, *CDS-β3-DNA.fa*, *Evolutionary tree proteins.fa*) and phenotypic raw data file (*Physiological data.txt*).

**b3-bifc.ab1, a-bifc-2.ab1,a-bifc-1.ab1**

Sequencing results of PpSnRK1α and PpSnRKβ3 interaction construction vectors identified by Bifc assay (sequence + peak plots).

**a-nluc-1.ab1,a-nluc-2.ab1,b3-cluc.ab1**

Sequencing results of PpSnRK1α and PpSnRKβ3 interaction construction vectors identified by Dual luciferase assay (sequence + peak plots).

**a-BD-1.ab1, a-BD-2.ab1,b3-AD.ab1**

Sequencing...