NPC1 promotes HTNV proliferation by controlling innate immune response

Host factors involved in hantaan virus(HTNV) infection was largely unknown, and we found cholesterol transporter NPC1 promotes HTNV proliferation. To dissect the mechanism by which NPC1 favors HTNV infection, bulk RNA-seq has been performed following HTNV infection in wild type and NPC1 knockout cells. In detail, wild type and NPC1 knockout HeLa cells were seeded on the 6-well plate for overnight culture, and HTNV(76-118 strain) was inoculated to the cells as the MOI=10, the mock infection cells were added to the same volume of Vero cell culture medium. We have four groups, including WT-Mock, WT-HTNV, KO-Mock and KO-HTNV, with three independent replicates each. All the samples were collected at 48 hours post infection by adding Trizol to lyse cells. The total RNA was extracted following the standard protocol, and the ployA-enriched RNA was used to construct the library and sequenced by the NovaSeq XPlus.