Tanscriptome profiling of cold exposure induced browning of white fat

We set two groups of 2-month-old C57BL6/J male mice at either thermoneutral temperature of 30℃ or under cold stimulation at 4℃ for one week. To identify events vital to beige fat activation, we performed RNA-seq using mice subcutaneous adipose tissue (iWAT) and focused on differentially-expressed genes characterized significant upregulation. The biege fat obtained from the mice with housed at either 30℃ or 4℃ for 7 days in a temperature-controlled incubator