Data from: Colonial choanoflagellate isolated from Mono Lake harbors a microbiome

Choanoflagellates offer key insights into bacterial influences on the origin and early evolution of animals. Here we report the isolation and characterization of a new colonial choanoflagellate species, <em>Barroeca monosierra</em>, that, unlike previously characterized species, harbors a stable microbiome. <em>B. monosierra</em> was isolated from Mono Lake, California and forms large spherical colonies that are more than an order of magnitude larger than those formed by the closely related <em>Salpingoeca rosetta</em>. By designing fluorescence <em>in situ</em> hybridization probes from metagenomic sequences, we found that <em>B. monosierra</em> colonies are colonized by members of the halotolerant and closely related Saccharospirillaceae and Oceanospirillaceae, as well as purple sulfur bacteria (Ectothiorhodospiraceae) and non-sulfur Rhodobacteraceae. This relatively simple microbiome in a close relative of animals presents a new experimental model for investigating the evolution of stable interactions among eukaryotes and bacteria. <br> This dataset contains the genome sequence of <em>B. monosierra</em>, as well as 24 associated bacterial genome sequences.<br> <br> The two ZIP archives whose names begin with "tree" contain all relevant data from the phylogenetic trees presented in Figure 1C and Figure S1. Each archive contains the following subdirectories: <br> input: input sequences in FASTA format polyA_removed: polyA tails removed by trimest (for protein coding genes only) aligned: sequence alignments by FSA trimmed: trimmed alignments by trimAl RAxML: input, run parameters and output of RAxML maximum likelihood tree reconstruction MrBayes: input, run parameters and output of MrBayes Bayesian tree reconstruction <br> The file Figure_2E.tree contains the tree from Figure 2E in Newick format.<br>