Expression data from rosette leaves of Arabidopsis thaliana wild type upon formaldehyde exposure

Formaldehyde is a toxic volatile organic compound and its mechanism of toxicity to plant has not yet been revealed. This experiment was designed to identify formaldehyde-responsible genes under exposure to low or high concentration of airborne formaldehyde for a short period of time. 7-weeks old Arabidopsis thaliana wild type (ecotype: Columbia) plants were exposed to gaseous formaldehyde at 1-2 ppm (low), 14-16 ppm (high), or less than 0.04 ppm (air control) at 24oC under light-condition for 150 minutes inside a chamber for formaldehyde exposure. Total RNA was isolated from rosette leaves of exposed plants and was applied to microarray analysis. We investigated into formaldehyde dose response on gene expression of Arabidopsis and tried to understand the toxic mechanisms of formaldehyde using an Affymetrix Arabidopsis genome array ATH-1. 7-week old Arabidopsis thaliana wild type (ecotype: Columbia) plants were exposed to gaseous formaldehyde at 1-2 ppm (low), 14-16 ppm (high), or less than 0.04 ppm (air control) at 24oC under light condition for 150 minutes inside of exposure chamber. When exposed under each condition, 3 plants were placed inside the exposure chamber, and these plants were treated as replications. In addition to treatment samples, non-treatment plants that were placed outside the chamber at 24oC under light-condition for 150 minutes until exposure were also prepared. Total RNA was isolated from rosette leaves of plants. In total, 12 samples were used in this experiment.