<b>Figure 5. Intact STING and MAVS signaling is required for Phase I HSV-1 gene expression.</b>

5A-B. Latently infected neurons were transduced with vectors expressing shSTING or shCTRL during latency. <br>5A. Relative expression of <i>ICP27</i> mRNA quantified by RT-qPCR at 0 and 18 hours post-reactivation (Phase I) with LY294002 (20μM). <br>5B. Relative expression of <i>Sting</i> mRNA quantified by RT-qPCR at 0 and 18 hours post-reactivation (Phase I) with LY294002 (20μM). <br>5C. Titers of infectious virus at 24 hours post-infection of Wild-type, STING KO, or MAVS KO ARPE-19 cells infected with KOS-SPA or KOS-UL98 at an MOI of 5 PFU/cell.  5D-E. Latently infected neurons were transduced with vectors expressing shMAVS or shCTRL during latency. <br>5D. Relative expression of <i>ICP27</i> mRNA quantified by RT-qPCR at 0 and 18 hours post-reactivation (Phase I) with LY294002 (20μM). <br>5E. Relative expression of <i>Mavs</i> mRNA quantified by RT-qPCR at 0 and 18 hours post-reactivation (Phase I) with LY294002 (20μM).   <br>5F. Relative expression of <i>ICP27</i> mRNA quantified by RT-qPCR at 0 and 18 hours post-reactivation (Phase I) following depletion of both STING and MAVS.

5A-B. 在潜伏感染阶段，采用表达短发卡RNA靶向STING（shSTING）或对照短发卡RNA（shCTRL）的载体对潜伏感染神经元进行转导。
5A. 采用20μM的LY294002处理后，于再激活后0小时与18小时（阶段I），通过实时定量聚合酶链反应（RT-qPCR）定量检测*ICP27* mRNA的相对表达水平。
5B. 采用20μM的LY294002处理后，于再激活后0小时与18小时（阶段I），通过实时定量聚合酶链反应（RT-qPCR）定量检测*Sting* mRNA的相对表达水平。
5C. 以感染复数（MOI）为5噬斑形成单位（PFU）/细胞的剂量感染KOS-SPA或KOS-UL98病毒后，于感染后24小时检测野生型、STING基因敲除（STING KO）与MAVS基因敲除（MAVS KO）ARPE-19细胞的感染性病毒滴度。
5D-E. 在潜伏感染阶段，采用表达短发卡RNA靶向MAVS（shMAVS）或对照短发卡RNA（shCTRL）的载体对潜伏感染神经元进行转导。
5D. 采用20μM的LY294002处理后，于再激活后0小时与18小时（阶段I），通过实时定量聚合酶链反应（RT-qPCR）定量检测*ICP27* mRNA的相对表达水平。
5E. 采用20μM的LY294002处理后，于再激活后0小时与18小时（阶段I），通过实时定量聚合酶链反应（RT-qPCR）定量检测*Mavs* mRNA的相对表达水平。
5F. 在同时沉默STING与MAVS基因后，于再激活阶段I的0小时与18小时，通过实时定量聚合酶链反应（RT-qPCR）定量检测*ICP27* mRNA的相对表达水平。