Comparative expression data between tumor epithelium of APCmin/+ FSPCre-PPARB -/- and PPARB flox/flox. Mus musculus

Stroma secretes complex regulatory signals, including reactive oxygen species, that are involved in mediating cancer development. Ablation of PPARB expression in fibroblastic cells results in modulation of oxidative stress signals that lead By means of gene expression analysis, we seek to identify key gene expression changes in tumor epithelial cells between FSPCre-PPARB -/- and PPARB flox/flox mice. Overall design: Expression profiling of FSPCre-PPARB KO tumor epithelium, FSPCre-PPARB KO tumor adjacent epithelium, PPARB floxed tumor epithelium, PPARB floxed tumor adjacent epithelium. Intestines were harvested from 25 week old mice on C57BL6/J APCmin/+ backgrounds and prepared for histological staining. Tumor and tumor adjacent epithelium were microdissected from stained histological slides using Zeiss PALM III laser disection microscope.