OMAP-26: Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Human Kidney with IMS-CODEX, v1.0

OMAP-26 was developed for CO-Detection by indEXing (CODEX) imaging of fresh frozen human kidney tissues, following<br>imaging mass spectrometry (IMS), as described in (Pham et al. 2025). The panel includes 22 antibodies and the nuclear<br>marker DAPI for image registration. This OMAP captures spatial context for all functional tissue units (FTUs) of the<br>nephron, as well as basement membranes, vasculature, lymphatics, stromal, and adaptive immune cells (B and T cells).<br>It does not include innate immune cells. The antibody panel in OMAP-26 significantly overlaps with other kidney panels<br>including OMAP-3 (Neumann and Farrow 2024), OMAP-9 (Barwinska et al. 2024), OMAP-14 (de Caestecker 2024), and OMAP-20<br>(Esselman 2024). All antibodies in OMAP-26 are also included in the expanded CODEX kidney panel OMAP-27<br>(https://doi.org/10.48539/HBM228.CPGH.632). OMAP-26 supports spatial mapping of structures and cell types listed in<br>the ASCT+B kidney table v1.6 (Jain et al. 2024). This panel was optimized and validated for use following MALDI IMS<br>imaging, supporting multimodal data integration. Additional details on sample preparation and antibody validation are<br>provided by the VU Biomolecular Multimodal Imaging Center and the Spraggins Research Group (2025).<br><br>**Bibliography:**<br><br>* Barwinska, Daria, Tarek M. El-Achkar, Angela R. Sabo, Michael J. Ferkowicz, Seth Winfree, and William S. Bowen. 2024.“9-Kidney-Codex (v1.3) Graph Data, v1.3.” https://lod.humanatlas.io/omap/9-kidney-codex/v1.3/.<br>* Caestecker, Mark de. 2024. “14-Kidney-Cycif (v1.0) Graph Data, v1.0.” https://lod.humanatlas.io/omap/14-kidney-cycif/v1.0/.<br>* Esselman, Allison. 2024. “20-Kidney-Ims-Cycif (v1.0) Graph Data, v1.0.” https://lod.humanatlas.io/omap/20-kidney-ims-cycif/v1.0/.<br>* Jain, Sanjay, M. Todd Valerius, Yongqun He, Tarek El-Achkar, Michael Eadon, and Jeffrey Spraggins. 2024. “Kidney (v1.6) Graph Data, v1.6.” https://lod.humanatlas.io/asct-b/kidney/v1.6/.<br>* Neumann, Elizabeth K., and Melissa Farrow. 2024. “3-Kidney-Codex (v1.3) Graph Data, v1.3.” https://lod.humanatlas.io/omap/3-kidney-codex/v1.3/.<br>* Pham, Thai, Lukasz Migas, Madeline E. Colley, Ellie Pringy, Anna Smith, Jamie Allen, Mark De Caestecker, et al. 2025. “Post MALDI IMS CODEX Staining and Imaging Fresh Frozen Tissue.” https://www.protocols.io/view/post-maldi-ims-codex-staining-and-imaging-fresh-fr-dzy277ye.<br>* VU Biomolecular Multimodal Imaging Center / Spraggins Research Group. 2025. “VU Biomolecular Multimodal Imaging Center / Spraggins Research Group - Research Workspace on Protocols.Io.” https://www.protocols.io/workspaces/BIOMIC.