Bulk RNA-Seq for MDSCs

Myeloid-derived suppressor cells (MDSCs) as a population of myeloid cells enriched in cancer patients with immunosuppressive function. Further studies have determined that MDSCs are comprised of two groups: polymorphonuclear-MDSCs (G-MDSCs) and monocytic-MDSCs (M-MDSCs). We used a microbeads kit (Miltenyi) to isolate G-MDSCs and M-MDSCs with markers Ly6c2-Ly6g+CD11b+ and Ly6c2+Ly6g-CD11b+, respectively. And then using bulk RNA-seq to anlysis the enrichment gene expression in G-or M-MDSC which from wild type, APCmin mutant adenoma and immune deffiency mice. Overall design: We isolate MDSCs from bone marrow (C57BL/6 WT mice), intestine (APCmin/+ mutant mice) and spleen (APCmin/+ mutant mice and BALB/c immune-diffency mice). Including bone marrow single cells (WT), (G+M)-MDSCs from spleen (BALB/c immune-diffency mice), Intestine single cells (APCmin/+ mutant mice), (G+M)-MDSCs from intestine (APCmin/+ mutant mice), (G+M)-MDSCs from spleen (APCmin/+ mutant mice), G-MDSCs from bone marrow (C57BL/6 WT), Spleen (APCmin/+ mutant mice and BALB/c immune-diffency mice) and M-MDSCs from bone marrow (C57BL/6 WT), Spleen (APCmin/+ mutant mice and BALB/c immune-diffency mice). Total 39 samples were analyzed including three replicates for each group.