Figure S1

Figure S1: Kidney innervation expands extensively from embryonic to postnatal stages. (A) Wholemount immunostain of an E14.5 kidney for TUBB3 (green), SMA (magenta), and CD31 (yellow). Axons track closely with the developing arteries despite the presence of an extensive CD31+ endothelial network. (B) Wholemount immunostain of a P0 kidney for TUBB3 (green), podocin (magenta), and LRP2 (yellow). The neuronal network continues to expand and navigate towards and around kidney structures. (C) Wholemount immunostain of a P0 kidney for TUBB3 (green), SMA (magenta), and podocin (yellow). The neuronal network has continued to grow as the vasculature expands and the last wave of nephrons differentiate.