FCS Autocorrelation analysis of ECFP-tubulin in PTK2 cells treated with microtubule depolymerizing agents.
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Each compound was added into culture medium at the following final concentration: nocodazole (0.3 μM), S-methyl DM1 (0.2 μM), demecolcine (1.6 μM) and vinblastine (1 μM). Average molecular number (ECFP-tubulin) and average residence time (inversely related to diffusion constant) were determined by fitting time autocorrelation curves (Fig. 4A) to a 3D diffusion model (see Methods).FCS Autocorrelation analysis of ECFP-tubulin in PTK2 cells treated with microtubule depolymerizing agents.
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2015-12-03
