Zfp36l2 mm10 Knockout eCLIP Tracks for UCSC Genome Browser

ZFP36L2 (zinc finger protein 36 like 2, C3H type-ZFP) is an RNA-binding protein targeting<br>transcripts rich in adenine-uridine elements (AREs). Previous transcriptomic analysis<br>suggested that ZFP36L2 displays a distinct transcript preference, depending on the tissue<br>of expression. However, this analysis was restricted to a few tissues. Here, we collected<br>RNA-seq data in six tissues and detected a remarkable transcript selectivity. Given that<br>ZFP36L2 accelerates the degradation of specific ARE-transcripts upon binding, we<br>obtained differential expression transcriptomic data on a Zfp36l2 knock-out mouse model<br>to delve into the mechanisms governing this tissue-specific targeting. Transcriptomic<br>analyzes of up regulated ARE-transcripts in lung, liver, bone marrow, spleen, kidney, and<br>ovary of the Zfp36l2-deficient mouse confirmed that there is high tissue preference in<br>ZFP36L2 targets. We observed only one common up regulated gene, Apol11b, among<br>these six different tissues. However, we do observe common trends, specifically an<br>enrichment in protein coding genes in the up regulated genes, consistent with these RBP<br>primarily targeting genes on their 3’ UTRs. Interestingly, we observed a significant<br>increase in the proportion of IG (immunoglobulin) genes being up regulated. We further<br>performed eCLIP (Enhanced Cross-Linking&amp;ImmunoPreciptation) on a mouse cell line,<br>MLTC-1 cells, to identify direct binding sites of ZFP36L2. AU-Rich Element score<br>(AREscore) analysis revealed enrichment in both up regulated genes and eCLIP peaks,<br>although some differences were observed in flanking residue composition. Our findings<br>provide new insights into the intricate regulatory network orchestrated by ZFP36L2,<br>opening avenues for exploring its potential roles in different tissues.