Transcriptional Responses to Elevated Temperature in Streptococcus agalactiae . Streptococcus agalactiae

In the transition from recto-vaginal colonizing organism to invasive pathogen, Streptococcus agalactiae (Group B Streptococcus, GBS) must adapt to changes in host temperature, including elevated temperatures due to host fever. To identify genes important to the survival of GBS in response to heat stress, transcriptional profiling was performed using DNA microarray analysis, comparing GBS grown at normal temperature (37˚C) to GBS exposed to elevated temperature (42˚C). Overall design: Two separate RNA samples were extracted for each condition. Three hybridizations were conducted total.