Homo sapiens raw sequence reads

In order to search for causative rare variants involved in the IgAN pathogenesis we performed an exome sequencing study on 24 individuals belonging to 8 families. For each pedigree we sequenced two affected individual and an internal negative control. Target regions were captured with the TruSeq Exome Enrichment and the illumina HiScanSQ system was used for sequencing. The magnitude of enrichment of captured ligation-mediated PCR products was determined using the Agilent 2100 Bioanalyzer. Next, each captured library was loaded onto the HiScanSQ platform, and paired-end sequencing was performed with read lengths of 101 bp. Image analysis was performed with default parameters of Illumina RTA v1.13 pipeline and demultiplexing was performed performed with CASAVA 1.8.2 (Illumina).