Glycine max cultivar:Williams82 Transcriptome or Gene expression

Soybean (Glycine max, cv Williams82) leaf petiole explants exposed to 25 ul/l ethylene for 0 to 72 h. Explants were prepared from 21 day-old greenhouse grown plants. Leaf abscission zones (LAZ) consisted of 2 mm of tissue collected below the leaf blade. The petioles (NAZ) consisted of approximately 3 to 4 mm of petiole tissue with the AZ removed. Explants and tissue were collected in February, March and April of 2013. Tissue and RNA were collected at USDA, Beltsville, MD (Mark L Tucker, Joonyup Kim and Ronghui Yang). Library construction and sequencing was completed at Univ of Cornell, Itheca, NY using a Illumina HiSeq 2000 (James J Giovannoni and Zhangjun Fei).

本研究选取了大豆（Glycine max, cv Williams82）叶片的叶柄外植体，在25 ul/l的乙烯气体中暴露0至72小时。外植体来源于温室中生长21天的植株。叶片脱落区（LAZ）包括位于叶片下方2毫米的组织。叶柄（NAZ）包括去除脱落区后约3至4毫米的叶柄组织。外植体和组织于2013年2月、3月和4月收集。组织及RNA样本由美国农业部Beltsville，MD（Mark L Tucker，Joonyup Kim和Ronghui Yang）提供。文库构建和测序工作在纽约州伊萨卡市的康奈尔大学完成，使用Illumina HiSeq 2000测序平台（James J Giovannoni和Zhangjun Fei）进行。