Next Generation Sequencing Facilitates Quantitative Analysis of PHB-knockdown and PHB-knockout Spermatocytes Transcriptomes

We analyzed the transcriptomic differences of cultured mouse spermatocytes (GC2 cells) stably transfected with PHB-targeting shRNA (called PHB KD GC2) from those with a control shRNA (called Ctrl GC2). Furthermore, we also analyzed the difference in the transcriptomes of spermatocytes between Phb conditional knock-out and control mice. Overall design: Examination of mRNAs in Ctrl GC2 cells, PHB KD GC2 cells and spermatocytes from Phb conditional knock-out and control mice. Three biological replicates of each sample were analyzed.