Cortical gene expression of WT, Grin1 knockdown, and Grin1 Cre rescue mice

The purpose of the study was to compare cortical transcript abundance between WT mice and Grin1 knockdown mice, and to determine whether transcript levels could be normalized in Grin1 Cre rescue mice (hemizygous for tamoxifen-inducible Rosa26 ERT2 Cre). All mice were treated with tamoxifen at 10 weeks of age until 12 weeks of age, and were euthanized at 15 weeks of age. Overall design: Methods: WT, GluN1, and GluN1Cre mice were used (all males, 4 per group). Total RNA isolated from cortex was used for RNAseq. Raw reads were processed on Galaxy server using up-to-date Tuxedo suite of programs. Differential gene expression determined with CuffDiff. Significant differences were determined by p<0.05, q<0.10