IL1-β effects on protein expression in lipid raft- and non-lipid raft fractions.

Changes in the expression pattern of proteins involved in signal transduction, apoptosis, and cell cycle were examined by antibody Array. Protein extracts (1 mg/ml) from INS-1 cells membrane lipid rafts and non lipid rafts, treated or not by IL1-β, were labeled with Cy3 and Cy5. Equal amounts of labelled proteins were incubated with antibodies doted on nitrocellulose-coated glass slides. The level of protein expression was quantified and the most representative changes are summarized on this Table.Up or down regulated membrane lipid raft and non lipid raft proteins after IL-1β treatment are presented in relative values recorded for the same proteins expressed in raft and non raft fraction in control condition (no IL-1β treatment). A 2-fold increase or decrease (bold) taken as cut-off value (positive or negative) is considered as significant.