m6A RNA Immunoprecipitaton and Sequencing of HTLV-1 infected cells

HTLV-1 is an oncogenic retrovirus responsible for adult T-cell leukemia (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Little is known regarding RNA chemical modifications of HTLV-1 RNA and how these affect viral biology and disease development. We investigated the role of N6-methyladenosine (m6A) in the context of HTLV-1 gene expression. Using methylated RNA immunoprecipitation and sequencing (MeRIP-Seq), we map sites of RNA modification to the 3' end of the viral genome that correspond with the regulatory region of the provirus.