Slamseq of whole mouse primary cortical neurons (mPCN) with perturbed mRNA degradation machinery
收藏NIAID Data Ecosystem2026-05-01 收录
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资源简介：
We perturbed mRNA degradation machinery in mouse primary cortical neurons (mPCN) and investigated the change in mRNA half-lives. We performed SLAMseq Metabolic RNA Labeling on Mutant mPCN line harbouring a ponasteroneA-inducible heterozygous dominant-negative Caf1 (dnCaf1) and on GFP-transfected mPCNs. The Slamseq technique provided snapshots of mRNA kinetics allowing to estimate mRNA half-lives.
创建时间：
2023-10-13
