Establishment of an induced pluripotent stem cell line from a female domestic ferret (Mustela putorius furo) with an X chromosome instability

The domestic ferret (ferret; Mustela putorius furo) is an important animal model for neuroscience and preclinical/veterinary medicine owing to its susceptibility to avian influenza and corona viruses. Nevertheless, there is a lack of in vitro ferret models, since immortal cell lines including induced pluripotent stem cells (iPSCs) of ferrets have been scarce. In this study, we established an induced pluripotent stem cell line from skin fibroblasts of a neonatal female ferret using a previously validated method in multiple mammalian species. The established line, fiPS-1, showed standard characteristics of pluripotency, but it showed an X chromosome instability characterized by the high emergence rate of aneuploid 39X cells from the original 40XX euploid cells. Overall design: RNA seq of ferret iPSCs