Ikaros-regulated microRNA networks in acute lymphoblastic leukemia

Purpose: miRNAs are important factors that are involved in the regulation cancer growth processes through the regulation of different biological processes. The purpose of the study was to investigate the differential expression pattern of miRNAs in IK1-induced B-ALL cells compared to controls. Methods: In this study, we used two human B-ALL cell lines with IZF1 deletions and induced IK1 expression or controls for 24 hours, and then RNA samples were prepared for the analysis of miRNAs profiles using the Genechip miRNA 4.0 arrays. The Genechip miRNA 4.0 array provides a comprehensive coverage that is designed to interrogate all mature miRNA sequences in miRBase Release 20. Result: The miRNAs expression microarray analysis showed that many miRNAs have been expressed differentially between IK1 and controls. These miRNAs might be involved in the different biological and physiological processes related to growth processes. Conclusion: miRNAs might be an active player Ikaros tumor suppression. 2 independent B-ALL cell-lines were used each with TET-inducible expression of IK1 or a empty vector control (CON). For each cell-line independent cultures were used as replicates, totalling 4 IK1 and 4 CON samples.