Development and evaluation of an indirect ELISA for detection of Teladorsagia circumcincta infection in sheep

The gastrointestinal helminth, <i>Teladorsagia circumcincta</i>, is one of the major health risk and production-limiting disease in small ruminant populations, particularly in temperate regions. With the increasing importance of the disease management and recruited anthelminic resistant types, accurate approaches are needed for diagnosis of the infection in the host. Because of uncertain results using faecal examinations, the ELISA method was indicated for detection of the nematode antigenic materials. Despite some promising results, problems were described in terms of the test specificity and the cross-reactions. The aim of this study was to evaluate worm somatic and excretory/secretory (ES) products using western blot analysis and an indirect ELISA for detecting <i>T. circumcincta</i> infection in sheep. Here, the file total score, includes the OD values and OD rates (ODR) obtained for the somatic and ES materials as well as the positive and negative pool controls.The second file (cross-reaction) shows the values and the graph obtained for the cross-reaction between ES and somatic materials of T. circumcincta and other worms examined. This file was prepared using Graphpad prism software.