Methylation profiling of pediatric choroid plexus tumors

Aggresome is a para nuclear inclusion body that functions as a storage compartment for misfolded proteins. Our previous work revealed the presence of aggresomes in pediatric choroid plexus tumors (CPT). CPTs are rare neoplasms comprised of three pathological subgroups; choroid plexus carcinoma (CPC), a grade III tumor, atypical choroid plexus papilloma (ACPP), a grade II tumor, and choroid plexus papilloma (CPP), a grade I tumor. In the current study, we aimed to investigate the prognostic value of aggresomes-positivity and its correlation to the pathological and molecular subtypes. The genome-wide methylation profile of 42 CPT pediatric samples was investigated using Illumina Infinium Methylation EPIC BeadChip array. Primary cohort comprised 42 tumor samples represented by 40 formalin-fixed-paraffin embedded (FFPE), 2 fresh frozen. 17 samples were represented by both FFPE and fresh frozen tissues. In total, we have 59 samples used for the Illumina Infinium 850K EPIC Methylation array.