Microbiota responses to mutations affecting NO homeostasis in A. thaliana (ITS dataset)

This bioproject contains the ITS amplicon dataset.Interactions between plants roots microorganisms are pivotal for plant growth and productivity. Several plant molecular mechanisms that shape these microbial communities have been identified. However, despite the numerous roles of nitric oxide (NO) in both plant and microbial processes, the importance of NO produced by plants for the associated microbiome remains elusive.Using a collection of Arabidopsis mutants overproducing NO or impaired in NO production, we investigated how altered NO homeostasis affects microbial communities both in the rhizosphere and in the roots, soil microbial activity as well as soil metabolites.We show that the rhizosphere microbiome was affected by the mutant genotypes, with the nox1 mutant overproducing NO and the nia1nia2 mutant impaired in NO production causing opposite shifts in bacterial and fungal communities compared to the wild type Col-0 in the rhizosphere and roots, respectively. The nia1nia2 and the nox1 mutants also exhibited distinctive soil metabolite profiles than those from the other genotypes while soil microbial activity did not differ between the mutants and the wild type Col-0.Our findings support our hypothesis that changes in NO production by plants can influence the plant microbiome composition with differential effects between fungal and bacterial communities.