Deep sequencing of M.tuberculosis beta-lactamase BlaC. E. coli

In this study, we used deep sequencing to determine relative fitness effect of four double site-saturation libraries of BlaC beta-lactamase in the presence of carbenicillin and the inhibitor clavulanic acid. Each library comprised variants of a residue known to be relevant for clavulanic acid resistance (130,132, 220 and 234) as well as residue 105. Libraries were screened at two temperatures (37 C and 30 C) in duplicate.