Estimates of oxidative stress and mutation frequency.

†Baer mutation accumulation (MA) line number from the Baer et al. (2005) experiment [28].‡Relative reactive oxygen species (ROS) levels expressed as means (standard error) of relative fluorescence units.*Indicates significantly different from N2 ancestor.§Means (standard error) of 8-oxo-7,8-dihydro-2′-deoxyguanosine, or 8-oxodG, are reported as ×109 damaged bases per nanogram of DNA.¶See Materials and Methods for calculations of point estimates of the frequencies of base substitutions (µBS) and G-to-T transversions (µG-TO-T).