Measurement of YopE production and survival in macrophages by different Y. pseudotuberculosis strains.
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(A) Static expression levels of YopE in the indicated strains as determined by immunoblotting analysis. Indicated bacteria strains were grown at 37°C in the presence of 2.5 mM CaCl2 for 2 h. Bacterial pellets were collected and analyzed by immunoblotting using antibodies specific for YopE. YopH levels are shown as loading controls. (B) Survival of intracellular bacteria was determined by CFU assay in infected macrophages (MOI of 10), as described in Figure 1. Results shown are the means from three independent experiments with duplicate infection wells. Error bars show standard deviations. **, P
创建时间：
2016-02-23
