Single-cell transcriptomic phenotyping combined with lineage tracing using paired B cell receptor repertoires identified both persistent antibody-secreting cell as well as memory B cell subsets after immunotherapy.

Disease relevant B cells during post-rituximab (RTX)relapseemerge from the unsuccessfuldepletion of pre-existing B cell clones; single-celltranscriptomic phenotyping combined with lineage tracing using paired B cell receptor repertoires identified both persistent antibody-secreting cell as well as memory B cellsubsets. Overall design: Examination of gene expression in B-lymphocytes utilizing 10X Genomics single-cell technology, deep sequenced on Illumina NovaSeq.