Estimated parameters (three-state model).

*not determined. The apparent rate constant, λ1′, is smaller than that of photobleaching (λb = 0.1 s−1), suggesting dissociation from the membrane rarely occurs when the molecule adopts state 1.#95% confidence interval for the estimates of diffusion coefficients.pj represents the ratio of the j-th subpopulation in the displacement data calculated at an arbitrary time in the trajectories.qj represents the initial probability that the molecule adopts the j-th state upon the onset of membrane association.The standard deviation of the measurement error, ε, was estimated from the trajectories by fitting MSD(Δt) from Δt to 2Δt.